Proposal ID: 964883
ICCS project ID: 63118100
Role: Partner
Acronym: UroPrint
Topic: FETOPEN-01-2018-2019-2020
Type of action: RIA
Call identifier: H2020-FETOPEN-2018-2020

UroPrint: Urinary bladder bioprinting for fully autologous transplantation

Duration in months: 48
Fixed keyword 1: Medical biotechnology
Fixed keyword 2: Technologies involving the manipulation of cells, tissues, organs or the whole organism
Fixed keyword 3: Regenerative medicine, Tissue engineering
Fixed keyword 4: Physical sciences, Optics, Laser beam forming & delivery
Free keywords: Urinary bladder engineering, autologous biometarials, Laser Induced Forward Transfer of cells, laser bioprinting, in vivo laser bioprinting

A number of conditions, including trauma, inflammation, incontinence, overactive bladder, renal impairments, neurological disorders (like spinal cord injury or spina bifida) and cancer, require bladder augmentation. For almost a century now, the majority of cystoplasties utilize bowel segments (enterocystoplasty). This, almost a century years old, gold standard practice bears numerous risks and complications affecting the majority of patients, thus compromising the quality of life while burdening the health care systems. This has fuelled efforts towards the development of engineered bladder tissue. Advancements in bioprinting technologies are increasingly employed in regenerative medicine but mostly in smaller and less complicated tissues. UroPrint proposes the use of Laser Induced Forward Transfer (LIFT) to generate bladder tissue for autologous transplantation that would meet the biological, mechanical and functional properties of human bladder. To this end, primary urothelial and smooth muscle cells will be obtained from healthy donors and expanded in fully Good Medical Practice compliant methodologies. These will be combined with novel natural autologous scaffold material obtained from platelet lysates. Then, a novel approach in the generation of bladder transplant will be utilized, combining intestine denudation and in vivo printing during surgery using a novel prototype LIFT printer that achieved high spatial resolution (<10m), high cell viability (>95%) single-urothelial cells.

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